The response of turkeys to dietary balanced protein during two periods of growth.

1. Two experiments were conducted to measure the response of growing turkeys to dietary protein content. In the first, 960 sexed British United Turkey (BUT 6) poults were used to measure the response to balanced protein from 3 to 6 weeks of age. In the second, 1440 sexed BUT and Hybrid Converter poults were raised from 14 to 17 weeks.2. In both experiments, six levels of dietary protein were fed, with feed intake, body and feather weight gain and changes in body composition measured. The levels of protein chosen ranged from 0.53 to 1.2 of the Aviagen requirements for growing turkeys.3. In the first experiment, six poults were sampled from each sex at the start of the experiment for carcass analysis, and four were sampled from each strain and sex in the second. At the end of each experiment, eight poults from each treatment were sampled. Body composition analyses were made on individual defeathered birds.4. Weight gain increased linearly with protein intake in the early period and exponentially in the later period. In both periods, feed intake decreased as protein content reduced.5. In the early period, body lipid content increased from 20.2 to 41.5 g/kg body weight, as dietary protein content decreased, but there was no change in the later period. Efficiency of utilisation of dietary protein declined linearly with an increase in dietary protein content, from 0.87 to 0.46 g/g in the first, and from 0.43 to 0.27 g/g in the later period.6. The inability of the growing turkey to increase feed intake on marginally limiting feeds may have been due to a genetic constraints to store excess energy consumed as body lipid, resulting in the observed decrease in feed intake as dietary protein content is reduced.

Evaluation of a mechanistic model that estimates feed intake, growth and body composition, nutrient requirements, and optimum economic response of broilers.

Efficient meat production is crucial in addressing global market demands and sustainability goals. Modeling production systems has gained worldwide attention, offering valuable insights for predicting outcomes and optimizing economic returns. In the poultry industry, researchers have developed mathematical models to predict animal performance and maximize profits. These models incorporate theories to explain real-world processes and enable future event predictions. One such model is the Broiler Growth Model (BGM), which serves as a predictive tool for estimating feed intake, growth, and body composition of broilers. The BGM takes into account the genetic potential of the broilers, the feed they are provided, and several constraining factors that may prevent the animal from achieving their genetic potential. To evaluate the BGM, a series of simulations were performed: (i) model behavior was evaluated by simulating the response of males and females from 22 to 35 d to feeds differing in dietary protein content and nutrient density; (ii) model prediction was evaluated using the results of a protein response trial conducted at UNESP in which six dietary protein levels were fed to male and female broilers over a 56 d period; and (iii) model optimization was used to maximize economic returns in the above trial. The model behaved as expected when feeds differing in protein content were fed, with feed intake per kg of BW increasing as protein level was decreased, resulting in lower gains and higher body lipid contents. Increasing nutrient density resulted in higher feed intake in the second level, followed by a reduction in feed intake in the highest nutrient feed. The simulated response to nutrient density resulted in increasing body lipid deposition as the nutrient density increased. In comparing the simulated and actual results of the protein response trial, the overall error of prediction was up to 15% for feed intake, BW, and body protein. The optimization routine allows the simulation of different economic scenarios, helping in decision-making. The Broiler Growth Model emerges as a valuable tool for the poultry industry, offering predictive capabilities and economic optimization potential. While minor discrepancies between simulated and actual results exist, the BGM holds significant promise for enhancing efficiency and profitability in broiler production, contributing to the broader goals of sustainable broiler meat production.

Update and evaluation of the egg production model in laying hens.

The egg production model (EPM) described here is a mechanistic and stochastic model that simulates the amino acid and energy requirements of laying hens. It takes into account their potential rate of laying, and egg weight over time, and the composition of the feed provided. The model predicts feed intake based on the assumption that hens will consume enough feed to reach their genetic potential. The environment is assumed to be non-limiting, although feed intake may be constrained by bulk capacity when bulky feeds are offered. The simulation model integrates a least-cost feed formulator, which enables the prediction of a feeding program that maximizes economic returns. A series of simulations were conducted to demonstrate the model behavior, external evaluation, and the economic optimization routine. The model behavior showed that as the energy content in the feed decreased, the simulated feed intake increased to compensate. Consequently, feeds with lower energy concentrations led to an overconsumption of nutrients, resulting in increased egg component weights (yolk and albumen). However, when the balanced protein was reduced while maintaining a constant energy-to-protein ratio, there was no change in egg components, consistent with published literature. The external evaluation indicated that feed intake, egg production, and egg weight were estimated with low error, and there was a similar trend observed between the estimated and observed data. This demonstrates the reliability of the model. Additionally, the paper provides a demonstration of how to use and interpret the results from the egg production model, including the economic optimization routine. The study found that a feed containing 7.8 g/kg of digestible lysine maximized economic returns. This optimization tool can be valuable in making nutritional decisions to optimize economic returns in an egg production system.

Review: When worlds collide - poultry modeling in the 'Big Data' era.

Within poultry production systems, models have provided vital decision support, opportunity analysis, and performance optimization capabilities to nutritionists and producers for decades. In recent years, due to the advancement of digital and sensor technologies, 'Big Data' streams have emerged, optimally positioned to be analyzed by machine-learning (ML) modeling approaches, with strengths in forecasting and prediction. This review explores the evolution of empirical and mechanistic models in poultry production systems, and how these models may interact with new digital tools and technologies. This review will also examine the emergence of ML and Big Data in the poultry production sector, and the emergence of precision feeding and automation of poultry production systems. There are several promising directions for the field, including: (1) application of Big Data analytics (e.g., sensor-based technologies, precision feeding systems) and ML methodologies (e.g., unsupervised and supervised learning algorithms) to feed more precisely to production targets given a 'known' individual animal, and (2) combination and hybridization of data-driven and mechanistic modeling approaches to bridge decision support with improved forecasting capabilities.

Dietary energy:protein ratio influences the efficiency of utilisation of dietary protein by growing turkeys.

1. The following experiment was designed to confirm that the efficiency of dietary protein utilisation (ep) can be reduced when the AMEn to digestible crude protein (DCP) ratio of the feed falls below a critical level. In addition, whether the efficiency would be improved at high DCP contents through the addition of oil or starch was determined.2. A 14d trial (starting at 21 d of age), using BUT 6 turkeys, was designed to measure the effects on ep of four feeds (12.1 MJ AMEn/kg) with AMEn:DCP ratios ranging from 45 to 72 MJ AMEn/kg, and two additional feeds with the same DCP as the highest protein feed, one with an AMEn:DCP ratio of 59, through the addition of canola oil, and the other with a ratio of 51 MJ/kg supplemented with both starch and oil. Twenty birds constituted each experimental unit, with two replications of each sex being used per dietary treatment.3. Ten birds per sex were sampled at the start of the trial and a further five per pen at the end for carcass analysis. Each carcass including feathers was ground and subsampled for water, protein and lipid determination.4. A split-line regression described ep on the four feeds unsupplemented with additional energy (R2 = 0.81) with breakpoint at 60.6 MJ AME/kg DCP, maximum ep at 0.736 g/g, and a slope of 0.017. No improvement in ep resulted from supplementing the high protein feed with oil or a mixture of oil and starch, but feed intake and protein and lipid retention increased significantly (P < 0.05).5. The argument that insufficient energy is available to enable high protein feeds to be utilised efficiently was not corroborated by the evidence from this trial.

Composition of feathers and pulp of two broiler genotypes.

1. Cobb and Ross broilers (200 of each sex and breed) were fed four phases of diets ad libitum formulated with balanced protein to match their amino acid requirements throughout growth. Ten birds per genotype were sampled and euthanised at two-weekly intervals from 14 to 112 d of age. All feathers were dry-plucked from each of the seven tracts (specific skin areas) and pulp (the centre of the feather filament) was removed from primary and secondary remiges.2. Daily losses of feathers were collected from an additional 20 individually-caged broilers of each breed. These feathers were separated into natal down, contour feathers, remiges and rectrices and then pooled by type, sex and genotype to quantify water and protein contents. Only those feathers collected from male Cobb 500 MX broilers were analysed for amino acid content.3. Amino acid contents of feathers from the seven tracts were measured only in Cobb males on days 1, 28 and 70; for pulp on days 28 and 70; and for the four types of moulted feathers.4. Protein content on a dry matter basis remained relatively constant over all ages and tracts during growth. Water content decreased with age in both sexes and genotype. Lysine and methionine content in feathers decreased with age while cystine, valine, leucine and serine increased. Lysine, methionine and histidine levels were higher in pulp than in mature feathers whereas cystine and valine were higher in mature feathers than in pulp.5. These results, together with information about moulting patterns in broilers, enabled the effects of age of the bird and of the type of feather, to be taken into account when determining the rate of deposition of amino acids in feathers.

Gut capacity of broiler breeder hens.

1. Broiler breeders are subjected to qualitative or quantitative feed restrictions to prevent obesity, which causes major health and welfare problems. Diluting their feed by adding inert or low nutrient, bulky materials can reduce obesity, but the capacity of the gut needs to be determined to apply this strategy successfully. Two trials were conducted to measure the bulk capacity of Ross 308 broiler breeders prior to and after the onset of lay. The trial was completely randomised, with nine individually-caged breeders, with each cage as a replicate, totalling 189 birds per trial2. Birds were given ad libitum access to one of 21 maize-soyabean based feeds, an undiluted control or progressive dilution (10, 20, 30 and 40%) with either cellulose fibre, rice husk, sand, vermiculite or sawdust. Feeds were analysed for density, crude-, acid detergent- and neutral detergent-fibre, water-holding capacity (WHC), cation-exchange capacity and oil-holding capacity.2. In general, feed intake (scaled to body weight0.67) increased and then declined as the proportion of each diluent increased. Intake increased linearly when rice hulls and sand were used as diluents.3. Water holding capacity was the most appropriate measure to define the gut capacity of broiler breeders.4. The trial data was used to estimate the maximum-scaled feed intake (SFImax) in broiler breeders, which was 240-56.1WHC + 4.34WHC2 g/kg0.67/d.

The response of reproducing Japanese quail to dietary valine.

1. A feeding trial was conducted to measure the responses of Japanese quail to dietary valine. In total, 280 Japanese quail were randomly assigned to eight treatments giving seven replicates (cage - 35 cm length, 35 cm width × 15 cm high). Experimental diets were formulated using a dilution technique to give a range dietary Val concentration (1.97 to 9.85 g/kg).2. Feed intake was maximised at 6.66 g Val/kg and above, but declined linearly below this level. Body weight reached a maximum of 170 g on 6.66 g Val/kg. Egg output peaked at 9.5 ± 0.3 g/bird/d with an egg weight of 11 g for the 6.66 g Val/kg diet. Rate of laying for the group that received the feed with the lowest Val content was close to zero (1.40%), but egg weight on this treatment was 70% of the maximum egg weight. Valine required per gram of egg output was estimated as 10.6 mg/g, whereas the maintenance requirement was 159 mg/kg body weight. Val required for maximum egg output was estimated in 154 mg/d.3. The marginal cost of Val in Brazil currently is negative below a level of 8.0 g/kg feed, which is above that required for maximum egg output. Consequently, Val cannot be regarded as a limiting amino acid currently, as the optimum economic intake exceeds the requirements of all the individuals in the population. The price of a quail egg weighing 11 g in Brazil at the time of the experiment was R$ 0.021. Even if the marginal revenue for these eggs was doubled to 0.4 c/g, there would be no reason to increase the intake of Val.

Prediction of maximum scaled feed intake in broiler chickens based on physical properties of bulky feeds.

1. A trial was conducted to investigate the capacity of broiler chickens to consume bulky feeds during three stages of growth. These phases were from 1 to 15 d, 16 to 30 d and from 31 to 45 d. 2. A basal feed was serially diluted (0%, 2.5%, 5.0%, 10% or 15%) with one of five diluents (cellulose fibre, sawdust, rice husk, sand or vermiculite) to produce 25 feeds which were supplied on an ad libitum basis to the birds in each phase. Cobb 500® strain chicks were used, and, within each phase, each feed was given to nine individually-caged birds, 225 in total, distributed in a completely randomised design. 3. Intake increased initially, and then declined, as the proportion of each diluent increased. The consumption of feeds that limited intake were directly proportional to metabolic body weight and so a scaled feed intake, expressed as g/BW0.67 per day, was calculated. There were large effects of feed type on intake, in the short term, with consumption of a bulky feed leading to higher intakes. 4. It was concluded the Water Holding Capacity (WHC) content of the feeds could be appropriate measurement of 'bulk' responsible for limiting intake and could be used to predict maximum feed intake capacities of broiler chickens fed bulky diets.

A description of the growth and moulting of feathers in commercial broilers.

1. Changes in feather length and weight and daily losses of down, contour feathers, remiges and retrices were studied in two commercial broiler strains to determine feather growth and moult in broilers up to 112 d of age. 2. Ten pens of 20 chicks for each sex x strain were fed adequate amounts of dietary protein in a four-phase feeding programme. Ten birds were sampled per genotype at 14, 28, 42, 56, 70, 84, 98 and 112 d. Feather loss was determined for individual birds caged within a nylon net. 3. All feathers were dry-plucked from each of seven tracts, with representative feathers from the capital-cervical, dorsopelvic and interscapular, pectoral and femoral tracts being randomly selected. Three rectrices of the dorsocaudal tract and three primaries and secondaries of the humeral-alar tract of the right wing were evaluated. 4. The length of the selected feathers was measured, and the feathers from each tract and from the whole bird were weighed. 5. A wide range of rates of maturing (0.0250-0.0907/d) and mature weights (9.62-52.9 g) were evident between sampled tracts. 6. Feather weight failed to predict some moults which were detected by the measurement of feather losses. From the weight data, moulting was evident only in the humeral-alar and dorsocaudal tracts, whereas, when daily losses were measured, contour feathers accounted for over 0.7 proportion of feather losses. Logistic equations adequately described the cumulative losses of down, contour feathers and remiges. 7. Feather loss needs to be considered when feather growth is determined from feather weight at different ages. 8. The rate of maturing (B) of feathers was numerically higher in Ross males and females than in the Cobb strain. In females, B was higher than in males (0.0483 vs. 0335/d) but the mature weight was lower (162 vs. 230 g).

The growth of turkeys 2. Body components and allometric relationships.

1. The relationships between the main components of the body and body protein among males and females of BUT 6 (BUT) and Hybrid Converter (HYB) turkey strains were examined. 2. The weights of breast meat, breast skin, drumstick meat, drumstick skin, thigh meat, thigh skin and wing-plus-skin as well as the head, neck, feet, blood, heart, liver and gizzard were measured at different stages of growth after which all components of each bird were minced together in order to determine the feather-free body protein weight of each bird sampled. Using the weights of the components and the protein content of each bird the allometric relationships between the components and body protein were determined and then compared for each strain x sex combination. 3. By excluding the breast weights at day-old and at 7 d, the remaining points produced an acceptable allometric relationship (R2 = 0.992). Thigh weight could be predicted for all strain x sex combinations using one allometric equation, as could drumstick skin weight. Breast and thigh skin weights differed between males and females. Females of the HYB strain exhibited heavier drumstick and wing-plus-skin weights at a given body protein weight than the other three strain x sex combinations. These differences in both the constant term and regression coefficient in the allometric equations between genotypes are probably due to differences in the amount of lipid that is deposited in these tissues. 4. Sexes differed in the allometric relationships for head, feet, heart and liver, while breeds differed in gizzard weight. A common relationship between the four genotypes could be used to predict the weight of blood in the carcass. 5. The allometric equations fitted to the data in this trial enable the accurate prediction of the weights of the different physical components given the weight of body protein.

The growth of turkeys 1. Growth of the body and feathers and the chemical composition of growth.

1. The potential growth of modern turkey genotypes was measured using male and female BUT 6 (BUT) and Hybrid Converter (HYB) strains. At one-day-old, 720 male and 960 female poults were randomly allocated to 48 littered pens in two houses with 30 males or 40 females of each strain per pen. Five feeds of decreasing protein content were fed to both sexes during the growing period. 2. Birds were weighed at 1, 7, 14, 21, 35, 56, 77, 119 d (males only), 126 d (females only) and 140 d (males only) and, on each occasion, six birds were selected and removed for carcass analysis. 3. Growth was similar in both strains, but the growth rate of females appeared to decline relative to the earlier trajectory after 70 d, and this tendency being greater in HYB females. 4. HYB birds of both sexes had more feathers than BUT to 77 d, but thereafter these differences were non-significant. A single allometry between feather and body protein weight was observed over all genotypes with no differences apparent between sexes within strains. The Gompertz curve adequately described the growth of body protein, water and ash. 5. Body lipid (g/100g bodyweight) declined in the early stages of growth. This might reflect an energy deficiency in the diet or might indicate that the present description of lipid growth does not apply in turkeys as it does in other species. 6. Whereas the description of body growth, feather growth and the chemical components of growth given in this paper may not fully reflect the true potential of the genotypes used, nevertheless they provide useful information regarding the latest genotypes available in the turkey industry. Some of the observations suggested that current performance might be improved if further research is conducted regarding dietary energy transactions.

The effect of dietary energy:protein ratio, protein quality and food allocation on the efficiency of utilisation of protein by broiler chickens.

1. Various theories have been proposed to explain the reduced performance of broilers when given feeds excessively high in protein, but a satisfactory solution to this problem had, up to now, not been found. Three experiments were conducted to test the hypothesis that the efficiency of utilisation of protein (ep) is a linear-plateau function of the ratio between the feed apparent metabolisable energy and digestible crude protein contents (AMEn:DCP) and that dietary protein quality, feed allocation and sex do not influence this relationship. 2. A 'linear-plateau' model successfully described the efficiency of protein utilisation (ep) as a function of AMEn:DCP in all three experiments. In Experiment 1, with both sexes being both ad libitum and control fed, the breakpoint was at 58.6 MJ AMEn/kg DCP. In Experiment 2, both sexes were fed balanced and unbalanced protein series, and at different rates, the slopes of the ascending part of the linear-plateau relationships for the different treatments were the same for all treatments (0.0204), and the inflection point was at 71 MJ AMEn/kg DCP. Using similar treatments in Experiment 3, the breakpoint for the balanced protein was 72 MJ/kg and for the unbalanced, 64, with a combined slope of 68 MJ AMEn/kg DCP. 3. The three experiments provide adequate evidence that ep is a linear-plateau function of the dietary AMEn:DCP ratio with a breakpoint of around 66.2 ± 1.98 MJ AMEn/kg DCP. Below this critical ratio, food intake declines as does protein and lipid retention. 4. That broilers, like pigs, exhibit an energy-dependent phase when high-protein feeds are offered is of practical importance when formulating pre-starter feeds for broilers and starter feeds for turkey poults as the ep of such feeds may well fall below the maximum due to the lack of dietary energy required to process the high dietary protein contained in such feeds resulting in poorer performance than expected.

Growth responses of entire and immunocastrated male pigs to dietary protein with and without ractopamine hydrochloride.

The interaction between dietary balanced protein, ractopamine (RAC) and Improvac (IMP) on the growth response of 120 individually penned male PIC© pigs was evaluated. The pigs entered the trial at 16 weeks of age and were assigned to 12 treatments using a 2×2×3 factorial design. Three balanced protein diets, formulated with standard illeal digestible lysine levels of 7.50 (low), 9.79 (medium) and 12.07 g/kg (high), were fed from 20 weeks. Improvac was administered at 16 and 20 weeks, the booster being given 4 weeks before slaughter. Ractopamine was supplemented at 10 mg/kg to the applicable treatments from 20 weeks. Live weight, backfat depth and food intake were measured on a weekly basis. Primary vaccination had no influence on the parameters measured. Immunocastration, RAC and higher protein diet treatments increased weight gain, but food intake was increased only with the use of IMP. Although weight gain increased in IMP-treated males, the increased feed intake resulted in their feed conversion efficiencies (FCE) being inferior to the intact controls. Feeding RAC only benefitted FCE when a high or medium protein diet was fed. Immunocastrates deposited more backfat after the booster vaccination than did entire males. Thus the cost of using RAC, IMP and of increasing dietary protein content needs to be weighed up against the additional revenue generated through the use of these interventions.

The response of weaned piglets to dietary valine and leucine.

Valine (Val) is considered to be the fifth-limiting amino acid in a maize-soyabean meal diet for pigs. Excess leucine (Leu) levels often occur in commercial diets, which may attenuate the effect of Val deficiency because of an increased oxidation of Val. The objective of the present experiment was to determine the effect of increasing concentrations of Leu on the response of young piglets to dietary Val. In all, 75 Large White×Landrace entire male pigs, 44 days of age and with a mean starting weight of 13.5 kg, were used. Three of these were sacrificed at the start to determine their mean initial chemical composition. A summit feed first limiting in Val was serially diluted with a non-protein diluent to produce a series of five digestible Val concentrations of 11.9, 10.1, 8.3, 6.6 and 4.8 g/kg, with a sixth treatment being added to test that the feeds were limiting in Val. Three identical Val series, each with six levels of Val, were supplemented with increasing amounts of Leu (23, 45 and 67 g/kg), thus 18 treatments in total. All pigs were killed at the end of the trial after 18 days for analysis of water, protein, lipid and ash in the carcass. The levels of Val and Leu and their interaction significantly influenced all the measurements taken in the trial. Daily gain in liveweight, water and protein, and feed conversion efficiency all increased with dietary Val content, whereas feed intake decreased as both Val and Leu contents increased. The deleterious effect of increased Leu on feed intake and growth was more marked at lower levels of Val. Supplementing the feed with the lowest Val content with additional Val largely overcame the effect of excess Leu. The efficiency of utilisation of Val for protein growth was unaffected by the level of Leu in the feed, the primary response to excess Leu being a reduction in feed intake. An intake of around 9 g Val/day yielded maximal protein growth during the period from 44 to 62 days of age in pigs of the genotype used in this trial.

Description of a model to optimise the feeding of amino acids to growing pullets.

1. A nutrition model is described that may be used to optimise the amino acid nutrition of laying-type pullets prior to the onset of lay. It is not a method of optimising lifetime laying performance. 2. The potential growth and composition of the body, feathers, ovary and oviduct are described from hatching to the age at which sexual maturity is attained, from which the daily amino acid and energy requirements for the average individual in the population can be calculated. 3. There are two parts to the approach used, the first being a description of the model itself and the second being a description of how the required information was gathered. A number of assumptions made in developing the model are discussed. 4. The rates of maturing of the body, feather-free body and body protein of the DeKalb pullets used were shown to be constant at 0.017/d and those of feathers at 0.02/d. These are considerably slower than those of the oviduct and ovary (0.139 and 0.084/d respectively). The ovary attained a higher mature weight (78.4 vs. 58.7 g) than the oviduct. 5. The age at which the growth of the reproductive organs is initiated in the model is defined by the user. The daily amount of threonine required to meet the requirements for maintenance and potential growth of the developing tissues and organs is calculated for each day of the growing period from hatching to the age at which the first egg is laid. A method of calculating the required daily concentration of threonine in the feed is described, from which a feeding programme may be derived. 6. For the model to produce an optimum economic feeding programme for a population of laying-type pullets, more information than is available from this study is required. These issues are discussed in the paper.

The rewards of using a modelling approach in directing poultry research.

The purpose of this paper was to demonstrate the advantages of using a good theory as the basis for designing and conducting research, using personal experience of developing a simulation model to predict food intake in laying hens and broiler breeders. To develop such a model, research projects were designed to measure, among others, the effect of lighting programmes on age at sexual maturity, changes in internal cycle length, egg and body component weights over time, effects of temperature on performance, and to determine whether these birds would make use of body lipid reserves as an energy source. Most of the experiments described here were conceived and conducted only because they were seen as a means of collecting information required for the development of empirical and mechanistic models, both of which have contributed to a better understanding of the birds themselves, as well as to the basis for predicting food intake in broiler breeders and laying hens. For those researchers seeking ideas for further study, there is no better way of generating such ideas than by first developing a theory of the subject to be studied, the greatest benefit from this approach being that such targeted research is bound to be new, innovative and useful.

Predicted optimum ambient temperatures for broiler chickens to dissipate metabolic heat do not affect performance or improve breast muscle quality.

An experiment was conducted to test the hypothesis that muscle damage in fast-growing broiler chickens is associated with an ambient temperature that does not permit the birds to lose metabolic heat resulting in physiological heat stress and a reduction in meat quality. The experiment was performed in 4 climate chambers and was repeated in 2 trials using a total of 200 male broiler chickens. Two treatments compared the recommended temperature profile and a cool regimen. The cool regimen was defined by a theoretical model that determined the environmental temperature that would enable heat generated by the bird to be lost to the environment. There were no differences in growth rate or feed intake between the two treatments. Breast muscles from birds on the recommended temperature regimen were lighter, less red and more yellow than those from the cool temperature regimen. There were no differences in moisture loss or shear strength but stiffness was greater in breast muscle from birds housed in the cool compared to the recommended regimen. Histopathological changes in the breast muscle were similar in both treatments and were characterised by mild to severe myofibre degeneration and necrosis with regeneration, fibrosis and adipocyte infiltration. There was no difference in plasma creatine kinase activity, a measure of muscle cell damage, between the two treatments consistent with the absence of differences in muscle pathology. It was concluded that breast muscle damage in fast-growing broiler chickens was not the result of an inability to lose metabolic heat at recommended ambient temperatures. The results suggest that muscle cell damage and breast meat quality concerns in modern broiler chickens are related to genetic selection for muscle yields and that genetic selection to address breast muscle integrity in a balanced breeding programme is imperative.

The response to dietary threonine in laying-type pullets during growth.

1. This study aimed to provide information on the response of laying-type pullets to dietary threonine (THR) during three periods of growth prior to the onset of lay. Different batches of Dekalb White pullets were used in three separate trial periods (from 4 to 6, 8 to 11 and 13 to 16 weeks of age) using 8 dietary THR concentrations in each period, using a completely randomised design, and with each treatment being replicated 6 times, using 15 birds per replication in period 1 and 8 birds in periods 2 and 3. In period 1 the THR content (THRc) ranged from 2.3 to 7.6 mg/g, in period 2 from 1.7 to 5.5 mg/g, and in period 3 from 1.4 to 4.7 mg THR/g feed. 2. Body weight gain, food intake and the deposition of protein and lipid in the feather-free body and in the feathers were measured in each period. Linear regressions were fitted to all data falling below the break point defined by the broken stick regression, to estimate the efficiency of utilisation of THR. The maximum protein growth rate was 4.0 ± 0.2, 5.3 ± 0.4 and 3.5 ± 0.5 g/d in periods 1, 2 and 3, respectively. 3. The efficiency of utilisation of dietary THR for THR deposition in each period was the same, at 0.85 ± 0.1 mg/mg. As dietary THRc decreased, the amount of body lipid deposition increased. 4. With this information, it is possible to determine the daily requirement for THR for the potential growth of body and feather protein in growing pullets.

The reproductive response of female ostriches to dietary protein.

A study was conducted with breeding ostriches over two consecutive breeding seasons to determine their response to different concentrations of a well-balanced dietary protein. Five concentrations of protein were fed to both females and males at an intake of 2.5 kg/bird d. The respective diets contained 75, 91, 108, 123 and 140 g protein/kg feed with energy held constant at 9.2 MJ metabolisable energy/kg feed. Egg production (mean ± SE, 39.1 ± 3.6 eggs/female/season) was unaffected by dietary protein concentration. Similarly, no significant trends were found for the number of unfertilised eggs (9.1 ± 1.8), dead-in-shell chicks (8.2 ± 1.3), the number of chicks hatched (19.5 ± 2.5) and change in the mass of females (-16.3 ± 10.2 kg). Egg weight decreased linearly as dietary protein content increased. Age of the ostrich female had a highly significant effect on the number of eggs laid, the number of chicks hatched, the number of dead-in-shell and infertile eggs produced per hen, as well as the mass change of female breeding birds, but did not affect the response of any of these variables to dietary protein content. It was concluded that ostriches do not benefit from dietary protein contents greater than about 75 g/kg when this is fed at a daily total feed intake rate of 2.5 kg/bird during the breeding season.